ok, this is very unlikely... D2O occurs in nature in very small quantities. separating D2O from H2O is a process requiring a lot of electricity, which is why the process is usually associated with hydroelectric plants. i've seen people on youtube enrich water, but in small quantities (a gallon or less). the process could be useful if you're doing experiments with a Farnsworth Fusor.
i live in Colorado, and as far as i can tell, most runoff from houses goes into the lawn, although this could be different in newer construction.
the OP should have some way of testing water for bacteria, unless the water has already been treated with chlorine or ozone. there are also water sterilizers that run the water past intense UV lamps.