As already mentioned, each neon needs it's own current limiting resistor - they are rather like LED's in that way.
For that matter, why not just use LED's? - better light, longer life, no high voltages required - it seems pointless using poorer old technology that will cost a LOT more to build, simply for the sake of using something old you have.
But assuming you want to?, if using two neons per segment, then you can put those two in series with a single limiting resistor - BUT you need an even higher voltage to supply them. Neons usually need between 60V and 90V, so it soon adds up if putting them in series.