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Why have you left R5 in place, and randomly changed R7 to a ludicrous 1 ohm, and R10 to 500 ohm?.OK, I followed your suggestions from post #20. I am getting pulses on HV_out of 3KV and 1.5mA at 43KHz.
Do you realize that you don't have a spice model for Q5? I'm guessing you got that error message, and ignored it, so LTSpice uses a VERY basic model. This model has a beta of 100 at any collector current, and it has nothing to reduce switching speed; no capacitance, no storage time, no ft. Real high power bipolar transistors generally have very slow switching speeds. In effect, the generic model is a current-controlled switch. It does have a base-emitter diode, but that only serves to slightly reduce the base current.
I could not find a spice model for Q5 (FLJ9620). You could use a different transistor. In your simulation, the collector voltage is only reaching 50V, but I suspect it will go higher if you ever get it to work...
You can't damage a transistor in a simulation.
EDIT: Have you considered a MOSFET? Seems to me that would be a better choice.
It is rise time that matters not the switching rate.200KHz is only a very low frequency, any transistor should be perfectly fine there.
You didn't say "20KV 5mA 13KHz", but it was a comment on the schematic that you posted in #18.No, that was a bad circuit and I didn't say that.